The United States has failed to qualify for the 2018 World Cup.
The US lost 2-1 to Trinidad and Tobago on Tuesday night while both Honduras and Panama won their games to pass the Americans in the CONCACAF standings.
Panama scored a hugely controversial goal to draw level with Costa Rica before scoring in the 87th minute to win 2-1 and earn the region's final automatic qualifying spot, while Honduras advanced to the intercontinental playoff against Australia by beating Mexico 3-2.
Panama finished in third place in the CONCACAF Hexagonal table with 13 points from 10 games, ahead of Honduras on goal difference, while the US ended with 12.
"We failed on the day. No excuses," said US coach Bruce Arena, who took responsibility for the failure to qualify. "We failed today. We should have walked off this field with at least a point."
Trinidad got on the scoreboard first when Omar Gonzalez deflected a 17th-minute cross that looped over Tim Howard and into the US goal.
It was 2-0 in the 36th minute when Alvin Jones smashed a swerving shot from the right flank past Howard to give the hosts a 2-0 advantage at the break.
Christian Pulisic gave the US the perfect start to the second half, firing home from just outside the penalty area just after kickoff to make it 2-1.
The US frantically chased another goal in the closing minutes, but it never came, which means Arena's team is the first American group since 1986 to not make the World Cup.